led fluorescent lamp Search Results


fluorescent lamp  (Excelitas corp)
96
Excelitas corp fluorescent lamp
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Fluorescent Lamp, supplied by Excelitas corp, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent lamp/product/Excelitas corp
Average 96 stars, based on 1 article reviews
fluorescent lamp - by Bioz Stars, 2026-05
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fluorescence microscope  (Nikon)
96
Nikon fluorescence microscope
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescence microscope/product/Nikon
Average 96 stars, based on 1 article reviews
fluorescence microscope - by Bioz Stars, 2026-05
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colibri led fluorescence lamp  (Carl Zeiss)
90
Carl Zeiss colibri led fluorescence lamp
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Colibri Led Fluorescence Lamp, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/colibri led fluorescence lamp/product/Carl Zeiss
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fluorescent lamps 1 godox led lamps 2 relio2 led lamps 3 δe00 mean  (Osram Sylvania)
86
Osram Sylvania fluorescent lamps 1 godox led lamps 2 relio2 led lamps 3 δe00 mean
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Fluorescent Lamps 1 Godox Led Lamps 2 Relio2 Led Lamps 3 δe00 Mean, supplied by Osram Sylvania, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
fluorescent lamps 1 godox led lamps 2 relio2 led lamps 3 δe00 mean - by Bioz Stars, 2026-05
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led lamp bx60 fluorescence microscope  (Evident Corporation)
90
Evident Corporation led lamp bx60 fluorescence microscope
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Led Lamp Bx60 Fluorescence Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/led lamp bx60 fluorescence microscope/product/Evident Corporation
Average 90 stars, based on 1 article reviews
led lamp bx60 fluorescence microscope - by Bioz Stars, 2026-05
90/100 stars
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axiolab epifluorescence microscope  (Carl Zeiss)
96
Carl Zeiss axiolab epifluorescence microscope
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Axiolab Epifluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axiolab epifluorescence microscope/product/Carl Zeiss
Average 96 stars, based on 1 article reviews
axiolab epifluorescence microscope - by Bioz Stars, 2026-05
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fluorescent lamp with blue led light islm-150x150bb  (CCS Inc)
90
CCS Inc fluorescent lamp with blue led light islm-150x150bb
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Fluorescent Lamp With Blue Led Light Islm 150x150bb, supplied by CCS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescent lamp with blue led light islm-150x150bb/product/CCS Inc
Average 90 stars, based on 1 article reviews
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800 nm-laser light psu-iii-led  (Laserglow Technologies)
90
Laserglow Technologies 800 nm-laser light psu-iii-led
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
800 Nm Laser Light Psu Iii Led, supplied by Laserglow Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/800 nm-laser light psu-iii-led/product/Laserglow Technologies
Average 90 stars, based on 1 article reviews
800 nm-laser light psu-iii-led - by Bioz Stars, 2026-05
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compact fluorescent lamps (cfls)  (EcoDesign Inc)
90
EcoDesign Inc compact fluorescent lamps (cfls)
Acute EtOH effects on cholinergic signaling. A <t>Fluorescent</t> image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.
Compact Fluorescent Lamps (Cfls), supplied by EcoDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/compact fluorescent lamps (cfls)/product/EcoDesign Inc
Average 90 stars, based on 1 article reviews
compact fluorescent lamps (cfls) - by Bioz Stars, 2026-05
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Image Search Results


Acute EtOH effects on cholinergic signaling. A Fluorescent image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.

Journal: bioRxiv

Article Title: Ethanol inhibits dorsomedial striatum acetylcholine release

doi: 10.1101/2025.05.30.656893

Figure Lengend Snippet: Acute EtOH effects on cholinergic signaling. A Fluorescent image of a striatal slice from a ChAT-Cre x Ai14 mouse. B CIN Cell-attached electrophysiology schematic. C Representative traces showing the acute effect of 40 mM EtOH on CIN firing rate. D Time course of 40mM EtOH (or ACSF control) effect on CIN firing rates (EtOH shaded region of graph). Control n=3 slices, EtOH n=5 slices. E Summary graph of 40 mM EtOH effect on CIN firing rate. F Schematic of ChR2 virus infusion in dorsal striatum and ChR2 expression. G Light activation of ChR2 causes ACh-driven DA release. H Representative traces showing the acute effect of 50 mM EtOH and 20 µM mecamylamine on ACh-driven DA release. I Time course of 50mM EtOH effect on ACh-driven DA release (EtOH shaded region of graph). n=16 slices. J Summary graph of 50mM EtOH effect on ACh-driven DA release. K Schematic of iAChSnFR virus infusion in dorsal striatum and iAChSnFR expression. L Microscope photometer setup for BSP recordings. M Representative traces showing the acute effect of 40 mM EtOH on evoked ACh release. N Time course of 40mM EtOH effect on evoked ACh release (EtOH shaded region of graph). n=5 slices. O Summary graph of 40mM EtOH effect on evoked ACh release. P Schematic of iAChSnFR (or NULL) virus infusions into dorsal striatum for in vivo fiber photometry experiments and fiber optic probe implant. Q Representative expression of iAChSnFR or NULL virus. R Schematic of open field fiber photometry setup for in vivo fiber photometry recordings. S Time course graph of ACh release following 2 g/kg EtOH (ip) administration. T Summary EtOH effect on in vivo dorsal striatal ACh release. *p<0.05, ***p<0.001. Error bars represent the SEM.

Article Snippet: The microscope consisted of a photometer with an 814 photomultiplier (Horiba, PTI D-104, Irvine, CA) fixed with a GFP filter (Semrock BrightLine, GFP-4050A-OMF-ZERO) and a fluorescent lamp (Excelitas Technologies, X-Cite XYLIS LED XT720S).

Techniques: Control, Virus, Expressing, Activation Assay, Microscopy, In Vivo